مبانی نظری و پیشینه پژوهشی بررسی تیتر آنتی بادی حاصل از واکسن Tri-Reo در فارم های مادر گوشتی بروش ELISA

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فهرست محتوا

فصل دوم : روش تحقيق 18

2-1-  روش تحقيق 18

2-1-1-برنامه واکسیناسیون 19

2-2-آزمايش ELISA 19

فهرست منابع: 35

منابع لاتین: 35

–  روش تحقيق

در اين مطالعه 5 فارم مادر گوشتي، دارای شرایط مديريتي و تغذيه‌ای مشابه انتخاب گردیدند. در طی دوره پرورش برنامه های واکسیناسیون اعمال شده در تمامی گله ها ثبت گردید. جهت واکسیناسیون برعلیه عفونت رئوویروس از واکسن Tri-Reo شرکت فایزر استفاده گردید.

در هریک از فارم ها، قبل از واکسیناسیون، 4 و 8 هفته پس از اعمال واکسیناسیون و در سن 42 هفتگی جهت بررسی عیار آنتی‌بادی علیه رئوویروس، خونگیری به عمل آمد و پس از ارسال به آزمایشگاه با استفاده از آزمایش‌ ELISA عیار آنتی‌بادی اندازه گیری گردید.

فهرست منابع

-زرع کاری، ع، فیضی، ع، نیک‌پیران، ح، (1385)، ارزیابی تیتر آنتی‌بادی حاصل از واکسن کشته رئوویروس در فارم‌های مادر گوشتی به روش الایزا، پایان نامه دوره دکترای حرفه‌ای، دانشگاه آزاد اسلامی واحد تبریز.

2-حسینی، ر، نیک‌پیران، ح، فیضی، ع، (1388)، بررسی مقایسه‌ای تیتر آنتی‌بادی برعلیه واکسن‌های نیوکاسل، برونشیت و آرتریت ویروسی در 3 فارم مرغ مادر گوشتی با 2 برنامه واکسیناسیون متفاوت در استان آذربایجان غربی، پایان نامه دوره دکترای حرفه‌ای، دانشگاه آزاد اسلامی واحد تبریز.

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